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PurposepLenti-EF1A-MCP-EXTEN-RecT-NLS
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183827 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMCP-RecT_NLS
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SpeciesSynthetic
- Promoter EF1A
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer EF1A-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMCP-RecT was a gift from Le Cong (Addgene plasmid # 183827 ; http://n2t.net/addgene:183827 ; RRID:Addgene_183827) -
For your References section:
dCas9-based gene editing for cleavage-free genomic knock-in of long sequences. Wang C, Qu Y, Cheng JKW, Hughes NW, Zhang Q, Wang M, Cong L. Nat Cell Biol. 2022 Feb;24(2):268-278. doi: 10.1038/s41556-021-00836-1. Epub 2022 Feb 10. 10.1038/s41556-021-00836-1 PubMed 35145221