Skip to main content
Addgene

p-dCas9-SSAP-MS2-BB_BbsI
(Plasmid #183826)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183826 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCBH
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    U6-guideRNA-dCas9-T2A-EBFP
  • Species
    Synthetic
  • Insert Size (bp)
    6291
  • Promoter CBH

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer pLKO
  • 3′ sequencing primer BHGpA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-dCas9-SSAP-MS2-BB_BbsI was a gift from Le Cong (Addgene plasmid # 183826 ; http://n2t.net/addgene:183826 ; RRID:Addgene_183826)

  • For your References section:

    dCas9-based gene editing for cleavage-free genomic knock-in of long sequences. Wang C, Qu Y, Cheng JKW, Hughes NW, Zhang Q, Wang M, Cong L. Nat Cell Biol. 2022 Feb;24(2):268-278. doi: 10.1038/s41556-021-00836-1. Epub 2022 Feb 10. 10.1038/s41556-021-00836-1 PubMed 35145221
Related items:
Commonly requested with: