pLenti-CD74-ROS1 L2000V
(Plasmid #183815)

• Purpose: Express CD74-ROS1 fusion (C6;R34) harboring ROS1 L2000V mutation in mammalian cells
• Depositing Lab: Aaron Hata
• Publication: Lin et al Clin Cancer Res. 2021 May 15;27(10):2899-2909. doi: 10.1158/1078-0432.CCR-21-0032. Epub 2021 Mar 8.

Backbone

• Vector backbone: pLenti
• Backbone size w/o insert (bp): 7877
• Total vector size (bp): 9430
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: ROS Proto-Oncogene 1, Receptor Tyrosine Kinase
• Alt name: ROS1
• Species: H. sapiens (human)
• Insert Size (bp): 1488
• Mutation: L2000V
• GenBank ID: NP_002935
• Entrez Gene: ROS1 (a.k.a. MCF3, ROS, c-ros-1)
• Promoter: CMV
• Tag / Fusion Protein:
  • The fusion protein of CD74 exon 1-6 and ROS1 exon 34-43

Cloning Information for Gene/Insert 1

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7

Gene/Insert 2

• Gene/Insert name: CD74 molecule
• Alt name: CD74
• Species: H. sapiens (human)
• Insert Size (bp): 625
• GenBank ID: NM_001025159.3
• Entrez Gene: CD74 (a.k.a. CLIP, DHLAG, HLADG, II, Ia-GAMMA, p33)
• Promoter: CMV
• Tag / Fusion Protein:
  • The fusion protein of CD74 exon 1-6 and ROS1 exon 34-43

Cloning Information for Gene/Insert 2

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry