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Addgene

pEFBOS_CreIRESpuro
(Plasmid #183812)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183812 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322/pUC
  • Total vector size (bp) 8097
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cre recombinase
  • Alt name
    Cre
  • Species
    Bacteriophage P1
  • Insert Size (bp)
    1029
  • Tag / Fusion Protein
    • NLS

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the polyA region has ambiguous bases in the Addgene NGS result due to difficulties sequencing through this region (4940-5005 bp).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEFBOS_CreIRESpuro was a gift from Ed Stanley (Addgene plasmid # 183812 ; http://n2t.net/addgene:183812 ; RRID:Addgene_183812)

  • For your References section:

    A protocol for removal of antibiotic resistance cassettes from human embryonic stem cells genetically modified by homologous recombination or transgenesis. Davis RP, Costa M, Grandela C, Holland AM, Hatzistavrou T, Micallef SJ, Li X, Goulburn AL, Azzola L, Elefanty AG, Stanley EG. Nat Protoc. 2008;3(10):1550-8. doi: 10.1038/nprot.2008.146. 10.1038/nprot.2008.146 PubMed 18802436
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