pAAV-Gad65P-eGFP-3XNLS-WPRE-pA
(Plasmid
#183804)
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PurposeAAV vector to express nuclear localized eGFP from a Gad65 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183804 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeGFP-NLS
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SpeciesOther
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Tag
/ Fusion Protein
- NLS (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: This plasmid contains only one ITR. This is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-Gad65P-eGFP-3XNLS-WPRE-pA was a gift from Jonathan Ploski (Addgene plasmid # 183804 ; http://n2t.net/addgene:183804 ; RRID:Addgene_183804) -
For your References section:
Adeno-associated viral serotypes differentially transduce inhibitory neurons within the rat amygdala. de Solis CA, Hosek MP, Holehonnur R, Ho A, Banerjee A, Luong JA, Jones LE, Chaturvedi D, Ploski JE. Brain Res. 2017 Oct 1;1672:148-162. doi: 10.1016/j.brainres.2017.07.023. Epub 2017 Jul 29. 10.1016/j.brainres.2017.07.023 PubMed 28764932