pRET.IIS.IRES-EGFP
(Plasmid #1838)

• Purpose: (Empty Backbone)
• Depositing Lab: Philip Leder
• Publication: Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35.

Backbone

• Vector backbone: pRET
• Vector type: Mammalian Expression, Retroviral, Cre/Lox
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Alt name: retroviral RET construct
• Alt name: removable exon trap
• Alt name: Type II vector

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See scanned map (#3).
This is a modified RET-EGFP vector for reasonably high-titer virus production, up to ~4x10^5 cfu/ml. However, the selectivity for the intragenic integration would not be as high as that achieved by other RET vectors (Type I). An additional XbaI site between EGFP and GHpA prevents isolation of essential elements of the vector on a single XbaI-XbaI fragment; this vector is not suitable for transfection.
(Leder #C-1022)