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pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA
(Plasmid #183776)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183776 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-CAG-3xmiR122-WPRE-HGHpA
  • Backbone size w/o insert (bp) 5258
  • Total vector size (bp) 6311
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cre
  • Species
    Synthetic
  • Insert Size (bp)
    1053
  • Mutation
    NA
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA was a gift from Ben Deverman (Addgene plasmid # 183776 ; http://n2t.net/addgene:183776 ; RRID:Addgene_183776)

  • For your References section:

    A High-Efficiency AAV for Endothelial Cell Transduction Throughout the Central Nervous System. Krolak T, Chan KY, Kaplan L, Huang Q, Wu J, Zheng Q, Kozareva V, Beddow T, Tobey IG, Pacouret S, Chen AT, Chan YA, Ryvkin D, Gu C, Deverman BE. Nat Cardiovasc Res. 2022 Apr;1(4):389-400. doi: 10.1038/s44161-022-00046-4. Epub 2022 Apr 13. 10.1038/s44161-022-00046-4 PubMed 35571675
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