pAAV-CAG-SV40NLSf-GFP-3xmiR122-WPRE-HGHpA
(Plasmid #183775)

• Purpose: AAV genome with a CAG driven eGFP reporter with mR122 target sequence repeats to reduce transgene expression in hepatocytes.
• Depositing Lab: Ben Deverman
• Publication: Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-400. doi: 10.1038/s44161-022-00046-4. Epub 2022 Apr 13.

Backbone

• Vector backbone: pAAV-CAG-3xmiR122-WPRE-HGHpA
• Backbone size w/o insert (bp): 5257
• Total vector size (bp): 6055
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NLS-GFP
• Species: Synthetic
• Insert Size (bp): 798
• Mutation: NA
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • pAAV-CAG-SV40NLSf-GFP-3xmiR122-WPRE-HGHpA.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry