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pGEX-2T-CBP(1603-2678)
(Plasmid #183774)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183774 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-2T
  • Backbone size w/o insert (bp) 4948
  • Total vector size (bp) 8180
  • Modifications to backbone
    add NdeI site at 5' and NsiI site at 3'.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nej
  • Alt name
    nejire, CBRB-binding protein
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    3228
  • Entrez Gene
    nej (a.k.a. Dmel_CG15319, CBP, CBP/p300, CBP_, CG15319, CG15321, Cbp, Crbp, Dmel\CG15319, Dmel_CG15321, Nej, Nejire, P300, anon-WO0147981.11, anon-WO03040301.89, cbp, dCBP, dKAT3, dmCBP, l(1)G0112, l(1)G0350, l(1)G0470, nej CBP, p300, p300/CBP)
  • Promoter tac
  • Tag / Fusion Protein
    • GST

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site NsiI (not destroyed)
  • 5′ sequencing primer ATG ATC AAA ATG GAG ATCAAG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The cDNA of CBP was from Sarah Smolik

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-2T-CBP(1603-2678) was a gift from Peter Harte (Addgene plasmid # 183774 ; http://n2t.net/addgene:183774 ; RRID:Addgene_183774)

  • For your References section:

    Histone demethylase UTX and chromatin remodeler BRM bind directly to CBP and modulate acetylation of histone H3 lysine 27. Tie F, Banerjee R, Conrad PA, Scacheri PC, Harte PJ. Mol Cell Biol. 2012 Jun;32(12):2323-34. doi: 10.1128/MCB.06392-11. Epub 2012 Apr 9. 10.1128/MCB.06392-11 PubMed 22493065
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