pGEX-2T-CBP(1603-2678)
(Plasmid #183774)

• Purpose: Express GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.
• Depositing Lab: Peter Harte
• Publication: Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34. doi: 10.1128/MCB.06392-11. Epub 2012 Apr 9.

Backbone

• Vector backbone: pGEX-2T
• Backbone size w/o insert (bp): 4948
• Total vector size (bp): 8180
• Modifications to backbone: add NdeI site at 5' and NsiI site at 3'.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: nej
• Alt name: nejire, CBRB-binding protein
• Species: D. melanogaster (fly)
• Insert Size (bp): 3228
• Promoter: tac
• Tag / Fusion Protein:
  • GST

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: NsiI (not destroyed)
• 5′ sequencing primer: ATG ATC AAA ATG GAG ATCAAG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The cDNA of CBP was from Sarah Smolik

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry