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pET-SU(Z)12(624-900)-H6
(Plasmid #183772)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183772 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-11d
  • Backbone size w/o insert (bp) 5670
  • Total vector size (bp) 6511
  • Modifications to backbone
    add 6 His tag at the C-terminus (after NsiI site)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SU(Z)12 residues 624-900
  • Alt name
    Su(z)12
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    831
  • Entrez Gene
    Su(z)12 (a.k.a. Dmel_CG8013, CG8013, Dmel\CG8013, SU(Z)12, SUZ12, Su(Z)12, Su(z), Su(z)[12], Suz(12), Suz12, l(3)76BDo, su(z)12)
  • Promoter T7
  • Tag / Fusion Protein
    • His tag at the C-terminus

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site NsiI (not destroyed)
  • 5′ sequencing primer CAT ATG TGT TCG CTC TTC GAC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pBluescript-SK-SU(Z)12 EST clone was from Drosophila Genome Resource Center

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-SU(Z)12(624-900)-H6 was a gift from Peter Harte (Addgene plasmid # 183772 ; http://n2t.net/addgene:183772 ; RRID:Addgene_183772)

  • For your References section:

    A 1-megadalton ESC/E(Z) complex from Drosophila that contains polycomblike and RPD3. Tie F, Prasad-Sinha J, Birve A, Rasmuson-Lestander A, Harte PJ. Mol Cell Biol. 2003 May;23(9):3352-62. doi: 10.1128/MCB.23.9.3352-3362.2003. 10.1128/MCB.23.9.3352-3362.2003 PubMed 12697833
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