pET-SU(Z)12(624-900)-H6
(Plasmid #183772)

• Purpose: Express of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.
• Depositing Lab: Peter Harte
• Publication: Tie et al Mol Cell Biol. 2003 May;23(9):3352-62. doi: 10.1128/MCB.23.9.3352-3362.2003.

Backbone

• Vector backbone: pET-11d
• Backbone size w/o insert (bp): 5670
• Total vector size (bp): 6511
• Modifications to backbone: add 6 His tag at the C-terminus (after NsiI site)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SU(Z)12 residues 624-900
• Alt name: Su(z)12
• Species: D. melanogaster (fly)
• Insert Size (bp): 831
• Promoter: T7
• Tag / Fusion Protein:
  • His tag at the C-terminus

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: NsiI (not destroyed)
• 5′ sequencing primer: CAT ATG TGT TCG CTC TTC GAC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pBluescript-SK-SU(Z)12 EST clone was from Drosophila Genome Resource Center

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry