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pET24TEV-tSHIP2
(Plasmid #183771)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183771 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET24b
  • Backbone manufacturer
    Novagen-EMD Millipore
  • Backbone size w/o insert (bp) 5309
  • Total vector size (bp) 8219
  • Modifications to backbone
    Tobacco Etch Virus (TEV) Cleavage Site inserted between HindIII and XhoI
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SHIP2
  • Alt name
    tSHIP2
  • Alt name
    INPPL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2920
  • Mutation
    includes aa18 to aa985 only
  • GenBank ID
    NM_001567
  • Entrez Gene
    INPPL1 (a.k.a. OPSMD, SHIP2)
  • Promoter T7 lac
  • Tags / Fusion Proteins
    • Tobacco Etch Virus (TEV) Cleavage site (C terminal on backbone)
    • 6HIS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET24TEV-tSHIP2 was a gift from William Kerr (Addgene plasmid # 183771 ; http://n2t.net/addgene:183771 ; RRID:Addgene_183771)

  • For your References section:

    LRBA Deficiency Can Lead to Lethal Colitis That Is Diminished by SHIP1 Agonism. Sudan R, Fernandes S, Srivastava N, Pedicone C, Meyer ST, Chisholm JD, Engelman RW, Kerr WG. Front Immunol. 2022 May 4;13:830961. doi: 10.3389/fimmu.2022.830961. eCollection 2022. 10.3389/fimmu.2022.830961 PubMed 35603158
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