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pS97splitRBP_S1-Enz
(Plasmid #183767)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183767 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKan S97Split
  • Backbone size w/o insert (bp) 6067
  • Total vector size (bp) 7456
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SHIP1
  • Alt name
    INPP5D
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1424
  • Mutation
    includes aa N393 to aa L864 only
  • GenBank ID
    NM_001017915
  • Entrez Gene
    INPP5D (a.k.a. SHIP, SHIP-1, SHIP1, SIP-145, hp51CN, p150Ship)
  • Promoter T7lac
  • Tags / Fusion Proteins
    • 6HIS (N terminal on backbone)
    • RBP (N-terminal) w/HRV3C cleavage site (N terminal on backbone)
    • RBP (C-terminal) w/HRV3C cleavage site (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Vector obtain from Stewart Loh, SUNY-Upstate Medical University
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pS97splitRBP_S1-Enz was a gift from William Kerr (Addgene plasmid # 183767 ; http://n2t.net/addgene:183767 ; RRID:Addgene_183767)

  • For your References section:

    LRBA Deficiency Can Lead to Lethal Colitis That Is Diminished by SHIP1 Agonism. Sudan R, Fernandes S, Srivastava N, Pedicone C, Meyer ST, Chisholm JD, Engelman RW, Kerr WG. Front Immunol. 2022 May 4;13:830961. doi: 10.3389/fimmu.2022.830961. eCollection 2022. 10.3389/fimmu.2022.830961 PubMed 35603158
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