Bxb1_AJMA
(Plasmid #183760)

• Purpose: Bxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703
• Depositing Lab: Richard Davis
• Publication: Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):100300. doi: 10.1016/j.crmeth.2022.100300. eCollection 2022 Oct 24.

Backbone

• Vector backbone: pBR322/pUC
• Total vector size (bp): 19499
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: ASAP2f
• Species: Synthetic
• Insert Size (bp): 1300

Gene/Insert 2

• Gene/Insert name: jRCaMP1b
• Species: Synthetic
• Insert Size (bp): 1200
• Tag / Fusion Protein:
  • NES

Gene/Insert 3

• Gene/Insert name: miRFP703
• Species: Synthetic
• Insert Size (bp): 950
• Tag / Fusion Protein:
  • Lck

Gene/Insert 4

• Gene/Insert name: ASAP2f
• Species: Synthetic
• Insert Size (bp): 1300

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry