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kiCAP-AAV-BI30
(Plasmid #183749)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183749 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57-Kan
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 9398
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AAV9 modified with 7mer insertion between amino acids 588 and 589 of VP1
  • Alt name
    BI30
  • Species
    Synthetic
  • Insert Size (bp)
    2232
  • Mutation
    K449R, and NNSTRGG inserted between 588 and 589 of AAV9 VP1
  • Promoter p41

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsiWI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    kiCAP-AAV-BI30 was a gift from Ben Deverman (Addgene plasmid # 183749 ; http://n2t.net/addgene:183749 ; RRID:Addgene_183749)

  • For your References section:

    A High-Efficiency AAV for Endothelial Cell Transduction Throughout the Central Nervous System. Krolak T, Chan KY, Kaplan L, Huang Q, Wu J, Zheng Q, Kozareva V, Beddow T, Tobey IG, Pacouret S, Chen AT, Chan YA, Ryvkin D, Gu C, Deverman BE. Nat Cardiovasc Res. 2022 Apr;1(4):389-400. doi: 10.1038/s44161-022-00046-4. Epub 2022 Apr 13. 10.1038/s44161-022-00046-4 PubMed 35571675
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