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Addgene

pHAGE-mNeptune2-SKL
(Plasmid #183729)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183729 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE
  • Backbone size w/o insert (bp) 7835
  • Total vector size (bp) 8582
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mNeptube2
  • Insert Size (bp)
    744
  • Promoter UbiC
  • Tag / Fusion Protein
    • SKL (peroxisomal targeting sequence 1) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.06.622258 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-mNeptune2-SKL was a gift from Jeffrey Gerst (Addgene plasmid # 183729 ; http://n2t.net/addgene:183729 ; RRID:Addgene_183729)

  • For your References section:

    Complementation of a human disease phenotype in vitro by intercellular mRNA transfer. Haimovich G, Dasgupta S, Ravi AG, Gerst JE. bioRxiv 2024.11.06.622258 10.1101/2024.11.06.622258
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