pHAGE-EGFP-SKL
(Plasmid
#183727)
-
PurposeFor exrpression of GFP targeted to peroxisomes
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 183727 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepHAGE
- Backbone size w/o insert (bp) 7835
- Total vector size (bp) 8564
-
Vector typeLentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameEGFP
-
Insert Size (bp)717
- Promoter UbiC
-
Tag
/ Fusion Protein
- SKL (peroxisomal targeting sequence 1) (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer -
- 3′ sequencing primer - (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.11.06.622258 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHAGE-EGFP-SKL was a gift from Jeffrey Gerst (Addgene plasmid # 183727 ; http://n2t.net/addgene:183727 ; RRID:Addgene_183727) -
For your References section:
Complementation of a human disease phenotype in vitro by intercellular mRNA transfer. Haimovich G, Dasgupta S, Ravi AG, Gerst JE. bioRxiv 2024.11.06.622258 10.1101/2024.11.06.622258