pMXs-Sig10(M2)-Fc-IG
(Plasmid
#183724)
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PurposeExpresses the fusion protein containing the extracellular part of the human Siglec-10 A108V mutant and the Fc region of human IgG1, and GFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183724 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMXs
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSiglec-10
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SpeciesH. sapiens (human)
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MutationA108V
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Tag
/ Fusion Protein
- Fc of human IgG1
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMXs-Sig10(M2)-Fc-IG was a gift from Takeshi Tsubata (Addgene plasmid # 183724 ; http://n2t.net/addgene:183724 ; RRID:Addgene_183724) -
For your References section:
A Guillain-Barre syndrome-associated SIGLEC10 rare variant impairs its recognition of gangliosides. Alborzian Deh Sheikh A, Gomaa S, Li X, Routledge M, Saigoh K, Numoto N, Angata T, Hitomi Y, Takematsu H, Tsuiji M, Ito N, Kusunoki S, Tsubata T. J Autoimmun. 2021 Jan;116:102571. doi: 10.1016/j.jaut.2020.102571. Epub 2020 Nov 19. 10.1016/j.jaut.2020.102571 PubMed 33223341