pMX-SHP1C-ires-hCD8
(Plasmid
#183720)
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PurposeExpresses the myc-tagged catalytic domains of wild-type SHP-1.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183720 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMXs
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMX-SHP1C-ires-hCD8 was a gift from Takeshi Tsubata (Addgene plasmid # 183720 ; http://n2t.net/addgene:183720 ; RRID:Addgene_183720) -
For your References section:
The Protein Tyrosine Phosphatase SHP-1 (PTPN6) but Not CD45 (PTPRC) Is Essential for the Ligand-Mediated Regulation of CD22 in BCR-Ligated B Cells. Alborzian Deh Sheikh A, Akatsu C, Abdu-Allah HHM, Suganuma Y, Imamura A, Ando H, Takematsu H, Ishida H, Tsubata T. J Immunol. 2021 Jun 1;206(11):2544-2551. doi: 10.4049/jimmunol.2100109. Epub 2021 May 14. 10.4049/jimmunol.2100109 PubMed 33990399