Inducible tdTomato
(Plasmid #183718)

• Purpose: tdTomato expression from dox-inducible promoter; plasmid encodes the TetR inducer
• Depositing Lab: Jeffrey Gerst
• Publication: Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.7554/eLife.83584.

Backbone

• Vector backbone: pLV
• Backbone size w/o insert (bp): 9300
• Total vector size (bp): 11986
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: tdTomato
• Insert Size (bp): 1431
• Promoter: TRE3GV

Cloning Information for Gene/Insert 1

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Gene/Insert 2

• Gene/Insert name: rTetR
• Insert Size (bp): 747
• Promoter: UbiC

Cloning Information for Gene/Insert 2

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • Inducible tdTomato.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.11.28.470233v2 for bioRxiv preprint.