tdTomato--β-actin CDS2
(Plasmid
#183714)
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PurposetdTomato with fragment nucleotides 398-818 of beta-actin coding sequence inserted in 3'UTR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183714 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHAGE
- Backbone size w/o insert (bp) 7838
- Total vector size (bp) 9690
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametdTomato with fragment (nucleotides 398-818) of mouse Actb coding dsequence
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SpeciesM. musculus (mouse)
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Insert Size (bp)1852
- Promoter UbiC
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer -
- 3′ sequencing primer - (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.11.28.470233v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
tdTomato--β-actin CDS2 was a gift from Jeffrey Gerst (Addgene plasmid # 183714 ; http://n2t.net/addgene:183714 ; RRID:Addgene_183714) -
For your References section:
Global analysis of contact-dependent human-to-mouse intercellular mRNA and lncRNA transfer in cell culture. Dasgupta S, Dayagi DY, Haimovich G, Wyler E, Olender T, Singer RH, Landthaler M, Gerst JE. Elife. 2023 May 30;12:e83584. doi: 10.7554/eLife.83584. 10.7554/eLife.83584 PubMed 37249209