pUbC-tdTomato
(Plasmid
#183710)
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PurposetdTomato expression from UbiC promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183710 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHAGE
- Backbone size w/o insert (bp) 7844
- Total vector size (bp) 9276
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametdTomato
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Insert Size (bp)1431
- Promoter UbiC
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer -
- 3′ sequencing primer - (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.11.28.470233v2 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUbC-tdTomato was a gift from Jeffrey Gerst (Addgene plasmid # 183710 ; http://n2t.net/addgene:183710 ; RRID:Addgene_183710) -
For your References section:
Global analysis of contact-dependent human-to-mouse intercellular mRNA and lncRNA transfer in cell culture. Dasgupta S, Dayagi DY, Haimovich G, Wyler E, Olender T, Singer RH, Landthaler M, Gerst JE. Elife. 2023 May 30;12:e83584. doi: 10.7554/eLife.83584. 10.7554/eLife.83584 PubMed 37249209
