AbVec_IGHC
(Plasmid #183701)

• Purpose: Encode human IgG1 heavy chain constant region. For cloning variable region and mAb expression.
• Depositing Lab: David Nemazee
• Publication: human antibody expression plasmid (unpublished)

Backbone

• Vector backbone: AbVec
• Backbone manufacturer: adapted from Burton lab
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 6500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IGHG1
• Alt name: IgG1 heavy chain
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• GenBank ID: 3500
• Entrez Gene: IGHG1
• Promoter: SP6

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: SP6-F
• 3′ sequencing primer: SV40-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: adapted from Burton lab mAb expression plasmid

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry