pRET.IL.PGK-TK
(Plasmid #1837)

• Purpose: (Empty Backbone)
• Depositing Lab: Philip Leder
• Publication: Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35.

Backbone

• Vector backbone: pRET
• Vector type: Mammalian Expression, Retroviral, Cre/Lox
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Alt name: retroviral RET construct
• Alt name: removable exon trap
• Alt name: Type I vector

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See scanned map (#5).
This is a modified RET vector exclusively for the DEL.BANK project (JC Schimenti, the Jackson Lab): the PGK promoter for the HSV-TK gene is much stronger than the MC1 promoter in other RET vectors. Virus production is virtually impossible. Either the PGK promoter or PGK poly A signal seems to be very inhibitory for the transcription of the virus genome. You can easily excise the essential components as an XbaI-XbaI fragment.
(Leder #C-1024)