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pCRISPRa_all-in-one_Bip gRNA1
(Plasmid #183696)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183696 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF1
  • Vector type
    CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gRNA targeting chinese hamster Bip (Hspa5)
  • gRNA/shRNA sequence
    Unspecified
  • Species
    gRNA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid also expresses dCas9-VP64, MS2-p65-HSF1 activation domains as CRISPRa synergistic Activation Mediators (SAM), and mCherry

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRISPRa_all-in-one_Bip gRNA1 was a gift from Edward Avezov (Addgene plasmid # 183696 ; http://n2t.net/addgene:183696 ; RRID:Addgene_183696)

  • For your References section:

    Stress-induced protein disaggregation in the endoplasmic reticulum catalysed by BiP. Melo EP, Konno T, Farace I, Awadelkareem MA, Skov LR, Teodoro F, Sancho TP, Paton AW, Paton JC, Fares M, Paulo PMR, Zhang X, Avezov E. Nat Commun. 2022 May 6;13(1):2501. doi: 10.1038/s41467-022-30238-2. 10.1038/s41467-022-30238-2 PubMed 35523806
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