pCRISPRa_all-in-one
(Plasmid
#183695)
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Purpose(Empty Backbone) Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183695 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEF1
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Vector typeCRISPR
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid also expresses MS2-p65-HSF1 activation domains as CRISPRa synergistic Activation Mediators (SAM) and mCherry
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCRISPRa_all-in-one was a gift from Edward Avezov (Addgene plasmid # 183695 ; http://n2t.net/addgene:183695 ; RRID:Addgene_183695) -
For your References section:
Stress-induced protein disaggregation in the endoplasmic reticulum catalysed by BiP. Melo EP, Konno T, Farace I, Awadelkareem MA, Skov LR, Teodoro F, Sancho TP, Paton AW, Paton JC, Fares M, Paulo PMR, Zhang X, Avezov E. Nat Commun. 2022 May 6;13(1):2501. doi: 10.1038/s41467-022-30238-2. 10.1038/s41467-022-30238-2 PubMed 35523806
