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pET30_Halotag_K73T_v2
(Plasmid #183692)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183692 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ER Halo(K73T)
  • Species
    Bacterial
  • Mutation
    K73T in Halotag

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30_Halotag_K73T_v2 was a gift from Edward Avezov (Addgene plasmid # 183692 ; http://n2t.net/addgene:183692 ; RRID:Addgene_183692)

  • For your References section:

    Stress-induced protein disaggregation in the endoplasmic reticulum catalysed by BiP. Melo EP, Konno T, Farace I, Awadelkareem MA, Skov LR, Teodoro F, Sancho TP, Paton AW, Paton JC, Fares M, Paulo PMR, Zhang X, Avezov E. Nat Commun. 2022 May 6;13(1):2501. doi: 10.1038/s41467-022-30238-2. 10.1038/s41467-022-30238-2 PubMed 35523806
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