SP_H6_Halo_M21K_F86L_KDEL_pBABEpu
(Plasmid
#183691)
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PurposeRetroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloM21K_F86L)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183691 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBABEpu
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Vector typeRetroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameER Halo(M21K_F86L)
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SpeciesBacterial
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MutationM21K_F86L in Halotag
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SP_H6_Halo_M21K_F86L_KDEL_pBABEpu was a gift from Edward Avezov (Addgene plasmid # 183691 ; http://n2t.net/addgene:183691 ; RRID:Addgene_183691) -
For your References section:
Stress-induced protein disaggregation in the endoplasmic reticulum catalysed by BiP. Melo EP, Konno T, Farace I, Awadelkareem MA, Skov LR, Teodoro F, Sancho TP, Paton AW, Paton JC, Fares M, Paulo PMR, Zhang X, Avezov E. Nat Commun. 2022 May 6;13(1):2501. doi: 10.1038/s41467-022-30238-2. 10.1038/s41467-022-30238-2 PubMed 35523806