mRFP-FKBP-INPP5E(D556A)
(Plasmid #183678)

• Purpose: Recruitable human type IV 5-phosphatase enzyme (catalytically inactive)
• Depositing Lab: Sergio Grinstein
• Publication: Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038/s41556-022-00895-y. doi: 10.1038/s41556-022-00895-y.

Backbone

• Vector backbone: mRFP-C1
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: INPP5E
• Alt name: CPD4; CORS1; JBTS1; MORMS; PPI5PIV; pharbin
• Species: H. sapiens (human)
• Mutation: C641A, D556A
• Entrez Gene: INPP5E (a.k.a. CORS1, CPD4, JBTS1, MORMS, PPI5PIV, pharbin)
• Promoter: CMV
• Tag / Fusion Protein:
  • mRFP-FKBP12(1-108) (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-Fwd
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Derived from Addgene Plasmid #67515

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry