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NES-mCherry-PH-BTKx1
(Plasmid #183653)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183653 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BTK
  • Alt name
    AT; ATK; BPK; XLA; IMD1; AGMX1; IGHD3; PSCTK1
  • Species
    H. sapiens (human), X. laevis (frog)
  • Mutation
    Amino acids 2-170
  • Entrez Gene
    BTK (a.k.a. AGMX1, AT, ATK, BPK, IGHD3, IMD1, PSCTK1, XLA)
  • Promoter CMV
  • Tag / Fusion Protein
    • X. laevis map2k1.L(32-44)-mCherry (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CMV-Fwd, mCherry-Fwd
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NES-mCherry-PH-BTKx1 was a gift from Sergio Grinstein (Addgene plasmid # 183653 ; http://n2t.net/addgene:183653 ; RRID:Addgene_183653)

  • For your References section:

    Kinase-independent synthesis of 3-phosphorylated phosphoinositides by a phosphotransferase. Walpole GFW, Pacheco J, Chauhan N, Clark J, Anderson KE, Abbas YM, Brabant-Kirwan D, Montano-Rendon F, Liu Z, Zhu H, Brumell JH, Deiters A, Stephens LR, Hawkins PT, Hammond GRV, Grinstein S, Fairn GD. Nat Cell Biol. 2022 Apr 28. pii: 10.1038/s41556-022-00895-y. doi: 10.1038/s41556-022-00895-y. 10.1038/s41556-022-00895-y PubMed 35484249
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