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Addgene

pcDNA3.3-hAP1 420-440
(Plasmid #183643)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183643 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.3
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATP6AP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    63
  • Mutation
    truncated ATP6AP1 mutant; contains only the transmembrane domain (amino acids 420-440)
  • Entrez Gene
    ATP6AP1 (a.k.a. 16A, ATP6IP1, ATP6S1, Ac45, CF2, VATPS1, XAP-3, XAP3)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.3-hAP1 420-440 was a gift from Sheng-cai Lin (Addgene plasmid # 183643 ; http://n2t.net/addgene:183643 ; RRID:Addgene_183643)

  • For your References section:

    Low-dose metformin targets the lysosomal AMPK pathway through PEN2. Ma T, Tian X, Zhang B, Li M, Wang Y, Yang C, Wu J, Wei X, Qu Q, Yu Y, Long S, Feng JW, Li C, Zhang C, Xie C, Wu Y, Xu Z, Chen J, Yu Y, Huang X, He Y, Yao L, Zhang L, Zhu M, Wang W, Wang ZC, Zhang M, Bao Y, Jia W, Lin SY, Ye Z, Piao HL, Deng X, Zhang CS, Lin SC. Nature. 2022 Mar;603(7899):159-165. doi: 10.1038/s41586-022-04431-8. Epub 2022 Feb 23. 10.1038/s41586-022-04431-8 PubMed 35197629
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