pBoBi-hPEN2 D90A
(Plasmid
#183641)
-
PurposeLentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the C-terminus (lysosome lumen).
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 183641 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBoBi
- Backbone size w/o insert (bp) 8300
-
Vector typeLentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePEN2
-
SpeciesH. sapiens (human)
-
Insert Size (bp)303
-
Mutationresidues 90 of PEN2 mutated to alanine
-
Entrez GenePSENEN (a.k.a. ACNINV2, MDS033, MSTP064, PEN-2, PEN2)
- Promoter CMV
-
Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBoBi-hPEN2 D90A was a gift from Sheng-cai Lin (Addgene plasmid # 183641 ; http://n2t.net/addgene:183641 ; RRID:Addgene_183641) -
For your References section:
Low-dose metformin targets the lysosomal AMPK pathway through PEN2. Ma T, Tian X, Zhang B, Li M, Wang Y, Yang C, Wu J, Wei X, Qu Q, Yu Y, Long S, Feng JW, Li C, Zhang C, Xie C, Wu Y, Xu Z, Chen J, Yu Y, Huang X, He Y, Yao L, Zhang L, Zhu M, Wang W, Wang ZC, Zhang M, Bao Y, Jia W, Lin SY, Ye Z, Piao HL, Deng X, Zhang CS, Lin SC. Nature. 2022 Mar;603(7899):159-165. doi: 10.1038/s41586-022-04431-8. Epub 2022 Feb 23. 10.1038/s41586-022-04431-8 PubMed 35197629