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Addgene

psfaMs-Tn5
(Plasmid #183637)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183637 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-29b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    anti mouse secondary nanobody fused to Tn5
  • Alt name
    ms-Tn5
  • Species
    E. coli
  • Insert Size (bp)
    2088
  • Tag / Fusion Protein
    • anti-mouse nanobody

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The construct was ordered as a synthetic gene and cloned at Twist Bioscience

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.03.08.483459 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psfaMs-Tn5 was a gift from Goncalo Castelo-Branco (Addgene plasmid # 183637 ; http://n2t.net/addgene:183637 ; RRID:Addgene_183637)

  • For your References section:

    Multimodal chromatin profiling using nanobody-based single-cell CUT&Tag. Bartosovic M, Castelo-Branco G. Nat Biotechnol. 2023 Jun;41(6):794-805. doi: 10.1038/s41587-022-01535-4. Epub 2022 Dec 19. 10.1038/s41587-022-01535-4 PubMed 36536148
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