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Addgene

PET28-FnCas12a-EP16
(Plasmid #183636)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183636 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PET28
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FnCas12a-EP16
  • Species
    Synthetic
  • Mutation
    N607R, K613V, N617R, K180S, K660R, D616N
  • Tag / Fusion Protein
    • 6×His Tag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PET28-FnCas12a-EP16 was a gift from Yunzi Luo (Addgene plasmid # 183636 ; http://n2t.net/addgene:183636 ; RRID:Addgene_183636)

  • For your References section:

    Improved CRISPR-Cas12a-assisted one-pot DNA editing method enables seamless DNA editing. Wang L, Wang H, Liu H, Zhao Q, Liu B, Wang L, Zhang J, Zhu J, Bao R, Luo Y. Biotechnol Bioeng. 2019 Jun;116(6):1463-1474. doi: 10.1002/bit.26938. Epub 2019 Feb 11. 10.1002/bit.26938 PubMed 30730047
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