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Addgene

pGJJ055
(Plasmid #183622)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183622 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    NEB
  • Backbone size (bp) 2686
  • Modifications to backbone
    Reduced in size by deleting the LacZ-alpha. Modified to harbour a landing site with HindIII and AvrII restriction sites so that the CYC promoter and DHFR3 fused to any protein of interest could be cloned into it. Three synonymous mutations were introduced in the Ampicillin resistance cassette to remove BtsαI restriction sites and a T>C synonymous substitution was added in the Ampicillin resistance cassette gene of pGJJ003 to create an Nb.BbvCI restriction site.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGJJ055 was a gift from Ben Lehner (Addgene plasmid # 183622 ; http://n2t.net/addgene:183622 ; RRID:Addgene_183622)

  • For your References section:

    Mapping the energetic and allosteric landscapes of protein binding domains. Faure AJ, Domingo J, Schmiedel JM, Hidalgo-Carcedo C, Diss G, Lehner B. Nature. 2022 Apr;604(7904):175-183. doi: 10.1038/s41586-022-04586-4. Epub 2022 Apr 6. 10.1038/s41586-022-04586-4 PubMed 35388192
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