pGJJ055
(Plasmid #183622)

• Purpose: (Empty Backbone) ddPCA mutagenesis plasmid - Efficient mutant library cloning plasmid suitable for oligo synthesis, error prone PCR or nicking mutagenesis strategies.
• Depositing Lab: Ben Lehner
• Publication: Faure et al Nature. 2022 Apr;604(7904):175-183. doi: 10.1038/s41586-022-04586-4. Epub 2022 Apr 6.

Backbone

• Vector backbone: pUC19
• Backbone manufacturer: NEB
• Backbone size (bp): 2686
• Modifications to backbone: Reduced in size by deleting the LacZ-alpha. Modified to harbour a landing site with HindIII and AvrII restriction sites so that the CYC promoter and DHFR3 fused to any protein of interest could be cloned into it. Three synonymous mutations were introduced in the Ampicillin resistance cassette to remove BtsαI restriction sites and a T>C synonymous substitution was added in the Ampicillin resistance cassette gene of pGJJ003 to create an Nb.BbvCI restriction site.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pGJJ055.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry