pGJJ045
(Plasmid
#183621)
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Purpose(Empty Backbone) abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183621 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGD110
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Backbone manufacturerLehner Lab
- Backbone size (bp) 6319
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Modifications to backboneBarcode landing site (BsiWI and AvrII restriction sites) added upstream of the CYC promoter driving the expression of DHFR3. Substituted the CYC promoter of DHFR12 for the GPD promoter.
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGJJ045 was a gift from Ben Lehner (Addgene plasmid # 183621 ; http://n2t.net/addgene:183621 ; RRID:Addgene_183621) -
For your References section:
Mapping the energetic and allosteric landscapes of protein binding domains. Faure AJ, Domingo J, Schmiedel JM, Hidalgo-Carcedo C, Diss G, Lehner B. Nature. 2022 Apr;604(7904):175-183. doi: 10.1038/s41586-022-04586-4. Epub 2022 Apr 6. 10.1038/s41586-022-04586-4 PubMed 35388192
Map uploaded by the depositor.
