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Addgene

pGJJ045
(Plasmid #183621)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183621 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGD110
  • Backbone manufacturer
    Lehner Lab
  • Backbone size (bp) 6319
  • Modifications to backbone
    Barcode landing site (BsiWI and AvrII restriction sites) added upstream of the CYC promoter driving the expression of DHFR3. Substituted the CYC promoter of DHFR12 for the GPD promoter.
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGJJ045 was a gift from Ben Lehner (Addgene plasmid # 183621 ; http://n2t.net/addgene:183621 ; RRID:Addgene_183621)

  • For your References section:

    Mapping the energetic and allosteric landscapes of protein binding domains. Faure AJ, Domingo J, Schmiedel JM, Hidalgo-Carcedo C, Diss G, Lehner B. Nature. 2022 Apr;604(7904):175-183. doi: 10.1038/s41586-022-04586-4. Epub 2022 Apr 6. 10.1038/s41586-022-04586-4 PubMed 35388192
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