lentiCRISPRv2-7SK-Blast
(Plasmid
#183620)
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Purpose(Empty Backbone) lentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183620 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonelentiCRISPRv2-Blast (#83480)
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Backbone manufacturerMohan Babu
- Backbone size (bp) 12567
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Modifications to backboneReplaced hU6 promoter with modified 7SK promoter.
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Vector typeMammalian Expression, Lentiviral, CRISPR
- Promoter Modified 7SK
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byMohan Babu (Addgene plasmid # 83480)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
lentiCRISPRv2-7SK-Blast was a gift from Bill Sugden (Addgene plasmid # 183620 ; http://n2t.net/addgene:183620 ; RRID:Addgene_183620) -
For your References section:
Optimal LentiCRISPR-Based System for Sequential CRISPR/Cas9 Screens. Hutcheson RL, Hayes M, Sugden B. ACS Synth Biol. 2022 Jun 29. doi: 10.1021/acssynbio.2c00152. 10.1021/acssynbio.2c00152 PubMed 35767740
