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pPyCAG-mKate2-3xNLS-IRES-Pac
(Plasmid #183618)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183618 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPyCAG-IRES-Pac
  • Backbone size w/o insert (bp) 6421
  • Total vector size (bp) 7222
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mKate2-3xNLS
  • Species
    Synthetic
  • Insert Size (bp)
    801
  • Promoter CAG
  • Tag / Fusion Protein
    • 3xNLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer TGCTGGTTGTTGTGCTGT
  • 3′ sequencing primer CGCACACCGGCCTTATTCCA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPyCAG-mKate2-3xNLS-IRES-Pac was a gift from Sally Lowell (Addgene plasmid # 183618 ; http://n2t.net/addgene:183618 ; RRID:Addgene_183618)

  • For your References section:

    Bone morphogenic protein signalling suppresses differentiation of pluripotent cells by maintaining expression of E-Cadherin. Malaguti M, Nistor PA, Blin G, Pegg A, Zhou X, Lowell S. Elife. 2013 Dec 17;2:e01197. doi: 10.7554/eLife.01197. 10.7554/eLife.01197 PubMed 24347544
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