attB53_SNCB-_attB53
(Plasmid #183615)

• Purpose: RMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.
• Depositing Lab: Sally Lowell
• Publication: Malaguti et al Development. 2022 May 26. pii: 275525. doi: 10.1242/dev.200226.

Backbone

• Vector backbone: attB53-Pac-attB53 (Addgene 183610)
• Backbone size w/o insert (bp): 3307
• Total vector size (bp): 9218
• Vector type: Mammalian Expression ; RMCE
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: LaG17 GFP nanobody SynNotch tTA
• Species: Synthetic
• Insert Size (bp): 2223
• Tags / Fusion Proteins:
  • human CD8a signal sequence (N terminal on insert)
  • Myc (N terminal on insert)

Gene/Insert 2

• Gene/Insert name: TRE-mCherry-SV40pA
• Insert Size (bp): 1189

Gene/Insert 3

• Gene/Insert name: tagBFP
• Alt name: Blue fluorescent protein
• Insert Size (bp): 694

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The Myc-LaG17-SynNotch-tTA cassette was a kind gift of Dr Wendell Lim and Dr Leonardo Morsut (Addgene plasmid 79128). The PGK-tagBFP and TRE-mCherry cassettes were a kind gift of Dr Elise Cachat (University of Edinburgh).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry