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attB53-Pac-TRE-3xFlag-Neurog1-attB53
(Plasmid #183613)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183613 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    attB53-Pac-TRE-mCherry-attB53 (Addgene 183611)
  • Backbone size w/o insert (bp) 3830
  • Total vector size (bp) 4667
  • Vector type
    Mammalian Expression ; RMCE
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Neurog1
  • Alt name
    Ngn1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    837
  • GenBank ID
    NM_010896.3
  • Entrez Gene
    Neurog1 (a.k.a. AKA, Math4C, Neurod3, bHLHa6, ngn1)
  • Promoter TRE
  • Tag / Fusion Protein
    • 3xFlag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site MfeI (not destroyed)
  • 5′ sequencing primer CGTTTCCCGTTGAATATGGCTC
  • 3′ sequencing primer GAAATTTGTGATGCTATTGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    attB53-Pac-TRE-3xFlag-Neurog1-attB53 was a gift from Sally Lowell (Addgene plasmid # 183613 ; http://n2t.net/addgene:183613 ; RRID:Addgene_183613)

  • For your References section:

    SyNPL: Synthetic notch pluripotent cell lines to monitor and manipulate cell interactions in vitro and in vivo. Malaguti M, Migueles RP, Annoh J, Sadurska D, Blin G, Lowell S. Development. 2022 May 26. pii: 275525. doi: 10.1242/dev.200226. 10.1242/dev.200226 PubMed 35616331
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