pmROSA26-attP50-Neo-mKate2-3xNLS-attP50
(Plasmid #183609)

• Purpose: Targeting vector for mouse ROSA26 locus to create landing pad cell line. Introduces attP50 sites flanking a Neo gene driven by endogenous locus (confers G418 resistance) and a CAG-mKate2-3xNLS.
• Depositing Lab: Sally Lowell
• Publication: Malaguti et al Development. 2022 May 26. pii: 275525. doi: 10.1242/dev.200226.

Backbone

• Vector backbone: pRosa26-DEST-1lox
• Backbone size w/o insert (bp): 10524
• Total vector size (bp): 15024
• Vector type: Mouse Targeting
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Neo
• Insert Size (bp): 804

Gene/Insert 2

• Gene/Insert name: mKate2-3xNLS
• Insert Size (bp): 786
• Tag / Fusion Protein:
  • 3xNLS (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pmROSA26-attP50-Neo-mKate2-3xNLS-attP50.gb
• A portion of this plasmid was derived from a plasmid made by: The pROSA-Dest-1lox backbone was a kind gift from Dr Keisuke Kaji (University of Edinburgh).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry