pPyPGK-HA-EGFP-Myc-TM-IRES-Pac
(Plasmid
#183606)
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PurposeMammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183606 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPyPGK-IRES-Pac
- Backbone size w/o insert (bp) 5236
- Total vector size (bp) 6329
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP-TM
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SpeciesSynthetic
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Insert Size (bp)1093
- Promoter Mouse Pgk1
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Tags
/ Fusion Proteins
- Mouse IgGK signal peptide (N terminal on insert)
- HA (N terminal on insert)
- Myc (C terminal on insert)
- Human PDGFRB transmembrane domain (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CATTCTGCACGCTTCAAAAG
- 3′ sequencing primer GCATTCCTTTGGCGAGAG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThe HA-EGFP-Myc-TM cassette was a kind gift of Dr Luis Ángel Fernández (CNB-CSIC).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPyPGK-HA-EGFP-Myc-TM-IRES-Pac was a gift from Sally Lowell (Addgene plasmid # 183606 ; http://n2t.net/addgene:183606 ; RRID:Addgene_183606) -
For your References section:
SyNPL: Synthetic notch pluripotent cell lines to monitor and manipulate cell interactions in vitro and in vivo. Malaguti M, Migueles RP, Annoh J, Sadurska D, Blin G, Lowell S. Development. 2022 May 26. pii: 275525. doi: 10.1242/dev.200226. 10.1242/dev.200226 PubMed 35616331