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Addgene

cPE
(Plasmid #183549)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183549 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Backbone size w/o insert (bp) 5814
  • Total vector size (bp) 9213
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9 nickase (H840A), Reverse Transcriptase
  • Insert Size (bp)
    5813
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cPE was a gift from Wen Xue (Addgene plasmid # 183549 ; http://n2t.net/addgene:183549 ; RRID:Addgene_183549)

  • For your References section:

    A flexible split prime editor using truncated reverse transcriptase improves dual-AAV delivery in mouse liver. Zheng C, Liang SQ, Liu B, Liu P, Kwan SY, Wolfe SA, Xue W. Mol Ther. 2022 Mar 2;30(3):1343-1351. doi: 10.1016/j.ymthe.2022.01.005. Epub 2022 Jan 5. 10.1016/j.ymthe.2022.01.005 PubMed 34998953
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