AAV8-cPE-N
(Plasmid
#183538)
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PurposeDeliver a split compact Prime Editor in vivo
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183538 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneaav
- Total vector size (bp) 6274
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameN-terminal fragment of SpCas9 nickase (H840A)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV8-cPE-N was a gift from Wen Xue (Addgene plasmid # 183538 ; http://n2t.net/addgene:183538 ; RRID:Addgene_183538) -
For your References section:
A flexible split prime editor using truncated reverse transcriptase improves dual-AAV delivery in mouse liver. Zheng C, Liang SQ, Liu B, Liu P, Kwan SY, Wolfe SA, Xue W. Mol Ther. 2022 Mar 2;30(3):1343-1351. doi: 10.1016/j.ymthe.2022.01.005. Epub 2022 Jan 5. 10.1016/j.ymthe.2022.01.005 PubMed 34998953