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Addgene

pAAV-nEF Coff/Fon DREADD Gq-mCherry
(Plasmid #183534)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183534 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-nEF
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4605
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DREADD Gq-mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    3212
  • Mutation
    None
  • Promoter nEF

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer GTTTAAAGCTCAGGTCGAGA
  • 3′ sequencing primer GAATACCAGTCAATCTTTCAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-nEF Coff/Fon DREADD Gq-mCherry was a gift from Karl Deisseroth (Addgene plasmid # 183534 ; http://n2t.net/addgene:183534 ; RRID:Addgene_183534)
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