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pMYH9-D1424H-FLAG
(Plasmid #183513)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183513 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST-FLAG
  • Backbone manufacturer
    Agilent
  • Backbone size w/o insert (bp) 4401
  • Total vector size (bp) 10284
  • Modifications to backbone
    origianl vector pCMV-Tag2b, converted into a Gateway compatible vector: pDEST-FLAG
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MYH9
  • Alt name
    NMHC-II-A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5883
  • Mutation
    changed Aspartic acid 1424 to Histidine
  • GenBank ID
    NM_002473.6
  • Entrez Gene
    MYH9 (a.k.a. BDPLT6, DFNA17, EPSTS, FTNS, MATINS, MHA, NMHC-II-A, NMMHC-IIA, NMMHCA)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer GGGAGGTCTATATAAGCAGAG
  • 3′ sequencing primer TAATACGACTCACTATAGGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: The base at position 4200, relative to the Snapgene file, is a G instead of a T, resulting in a synonymous change p.Ala1143Ala

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMYH9-D1424H-FLAG was a gift from Emanuele Panza (Addgene plasmid # 183513 ; http://n2t.net/addgene:183513 ; RRID:Addgene_183513)

  • For your References section:

    Transfection of the mutant MYH9 cDNA reproduces the most typical cellular phenotype of MYH9-related disease in different cell lines. Panza E, Marini M, Pecci A, Giacopelli F, Bozzi V, Seri M, Balduini C, Ravazzolo R. Pathogenetics. 2008 Dec 1;1(1):5. doi: 10.1186/1755-8417-1-5. 10.1186/1755-8417-1-5 PubMed 19046415
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