pMYH9-WT-V5
(Plasmid #183512)

• Purpose: Gateway expression vector encoding wild type MYH9 cDNA with N-terminal V5 tag. For mammalian expression.
• Depositing Lab: Emanuele Panza
• Publication: Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 10.1186/1755-8417-1-5.

Backbone

• Vector backbone: pcDNA3.1/nV5-DEST
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5502
• Total vector size (bp): 11385
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MYH9
• Alt name: NMHC-II-A
• Species: H. sapiens (human)
• Insert Size (bp): 5883
• GenBank ID: NM_002473.6
• Entrez Gene: MYH9 (a.k.a. BDPLT6, DFNA17, EPSTS, FTNS, MATINS, MHA, NMHC-II-A, NMMHC-IIA, NMMHCA)
• Promoter: CMV
• Tag / Fusion Protein:
  • V5 (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: GGGAGGTCTATATAAGCAGAG
• 3′ sequencing primer: CAATGCGATGCAATTTCCTC

Resource Information

• Supplemental Documents:
  • pMYH9-WT-V5.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry