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Addgene

pDEST-FLAG
(Plasmid #183511)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183511 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-tag2B
  • Backbone manufacturer
    Agilent
  • Backbone size (bp) 6035
  • Modifications to backbone
    modification to a Gateway compatible vector by the introduction of a ccdB vector conversion cassette
  • Vector type
    Mammalian Expression
  • Promoter CMV
  • Selectable markers
    Neomycin (select with G418)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-FLAG was a gift from Emanuele Panza (Addgene plasmid # 183511 ; http://n2t.net/addgene:183511 ; RRID:Addgene_183511)

  • For your References section:

    Transfection of the mutant MYH9 cDNA reproduces the most typical cellular phenotype of MYH9-related disease in different cell lines. Panza E, Marini M, Pecci A, Giacopelli F, Bozzi V, Seri M, Balduini C, Ravazzolo R. Pathogenetics. 2008 Dec 1;1(1):5. doi: 10.1186/1755-8417-1-5. 10.1186/1755-8417-1-5 PubMed 19046415
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