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Addgene

pYUBcLIC-GFP
(Plasmid #183509)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183509 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYUBcLIC-GFP
  • Backbone manufacturer
    Matthew Bennett
  • Backbone size (bp) 5611
  • Vector type
    Bacterial Expression
  • Promoter T7 lac
  • Tags / Fusion Proteins
    • folding reporter GFP (C terminal on insert)
    • 10x His-tag (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7-for : TAA TAC GAC TCA CTA TAG GG
  • 3′ sequencing primer T7-rev: CTA GTT ATT GCT CAG CGG T
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pYUB vector backbone originates from pYUB28b (Addgene plasmid # 37277 ; http://n2t.net/addgene:37277 ; RRID:Addgene_37277).

Reference: Metabolic engineering of cofactor F420 production in Mycobacterium smegmatis. Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN. PLoS One. 2010 Dec 29;5(12):e15803. doi: 10.1371/journal.pone.0015803 PubMed 21209917
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LIC cassette together with folding reporter GFP and purification tag sequence originate from pREcLIC-GFP.

Reference: Geertsma, E., Poolman, B. High-throughput cloning and expression in recalcitrant bacteria. Nat Methods 4, 705–707 (2007). doi: 10.1038/nmeth1073 PubMed 17643108

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYUBcLIC-GFP was a gift from Martin Högbom (Addgene plasmid # 183509 ; http://n2t.net/addgene:183509 ; RRID:Addgene_183509)

  • For your References section:

    High-throughput strategy for identification of Mycobacterium tuberculosis membrane protein expression conditions using folding reporter GFP. Grave K, Bennett MD, Hogbom M. Protein Expr Purif. 2022 Oct;198:106132. doi: 10.1016/j.pep.2022.106132. Epub 2022 Jun 21. 10.1016/j.pep.2022.106132 PubMed 35750296
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