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pTRIP-MND-HsMAN1C1-1-630-cMycFlag-hPGK-GFP
(Plasmid #183505)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183505 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTRIP
  • Total vector size (bp) 12890
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    man1C1
  • Alt name
    class I alpha-mannosidase C1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1890
  • GenBank ID
    NM_020379 NM_020379.3
  • Entrez Gene
    MAN1C1 (a.k.a. HMIC, MAN1A3, MAN1C, pp6318)
  • Promoter MND
  • Tag / Fusion Protein
    • Myc-DDK (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer 5’CTCACTCGGCGCGATCTGGATCTGCCGCCGCGATCG3’ (Forward)
  • 3′ sequencing primer 5’CCCAACCCCGTGGGAATTCGTTAAACCTTATCGTCGTCATCCTTG3’ (Reverse)
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    EGFP
  • Insert Size (bp)
    239
  • Promoter hPGK

Cloning Information for Gene/Insert 2

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer 5’CACTCGGCGCGATCTGGATCCCGAATTCCCACGGGGTTG3’ (Forward)
  • 3′ sequencing primer 5’CACCATGGTGGCGACCGGTGGCTGGGGAGAGAGGTCGG3’ (Reverse)
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    man1c1: from pCMV6-HsMAN1C1-1-630-cMycFlag plasmid (Origene, Cat#: RC222009)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRIP-MND-HsMAN1C1-1-630-cMycFlag-hPGK-GFP was a gift from Olivier Guipaud (Addgene plasmid # 183505 ; http://n2t.net/addgene:183505 ; RRID:Addgene_183505)

  • For your References section:

    A role for endothelial alpha-mannosidase MAN1C1 in radiation-induced immune cell recruitment. Ladaigue S, Lefranc AC, Balde K, Quitoco M, Bacquer E, Busso D, Piton G, Depagne J, Dechamps N, Yamakawa N, Debusschere L, Han C, Allain F, Buard V, Tarlet G, Francois A, Paget V, Milliat F, Guipaud O. iScience. 2022 Nov 2;25(12):105482. doi: 10.1016/j.isci.2022.105482. eCollection 2022 Dec 22. 10.1016/j.isci.2022.105482 PubMed 36404925
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