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Addgene

pGEM4Z-Fluc
(Plasmid #183474)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183474 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM4Z
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2600
  • Total vector size (bp) 4400
  • Vector type
    Bacterial Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Firefly luciferase
  • Alt name
    Fluc
  • Species
    Photinus pyralis
  • Insert Size (bp)
    1829
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Sac I (not destroyed)
  • 5′ sequencing primer TATAATACGACTCACTATAGGGAGACAA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM4Z-Fluc was a gift from Chantal Pichon (Addgene plasmid # 183474 ; http://n2t.net/addgene:183474 ; RRID:Addgene_183474)

  • For your References section:

    Neutral Lipopolyplexes for In Vivo Delivery of Conventional and Replicative RNA Vaccine. Perche F, Clemencon R, Schulze K, Ebensen T, Guzman CA, Pichon C. Mol Ther Nucleic Acids. 2019 Sep 6;17:767-775. doi: 10.1016/j.omtn.2019.07.014. Epub 2019 Jul 30. 10.1016/j.omtn.2019.07.014 PubMed 31446119
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